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PVDF membranes were blocked with 5% skim milk at room temperature for 2 h and then incubated with primary antibodies at 4 C overnight, followed by incubation with secondary horseradish peroxidase-conjugated antibodies (1:1000, Sungene Biotech, China) for 1 h at room temperature

(Vienna) 130 (5), 627646

307 The system was used to evaluate 1,849 interactions between 43 Streptococcus pneumoniae proteins in quadruplicate, mapping a rich interaction network